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wt fvb mice  (Miltenyi Biotec)


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    Miltenyi Biotec wt fvb mice
    Wt Fvb Mice, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 595 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wt+fvb+mice/pmc12910057-228-20-24?v=Miltenyi+Biotec
    Average 97 stars, based on 595 article reviews
    wt fvb mice - by Bioz Stars, 2026-07
    97/100 stars

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    In vivo characterization of the full-length CP040 promoter packaged in the AAVDJ capsid (A) 1 × 10 13 vg/kg of AAVDJ-CP040 -eGFP or vehicle (1x PBS) was injected into the temporal vein of <t>wild-type</t> <t>FVB/NJ</t> neonatal mice (postnatal day 1–2). Necropsies were performed on the mice at six weeks of age, and tissue samples were analyzed for vg content and eGFP transcript levels. (B) Graph presenting vg copy numbers in different organs—cortex, cerebellum, kidneys, spleen, quadriceps, spinal cord, liver and heart. (C) Graph showing the fold change in e GFP transcript levels demonstrating promoter strength in the same organs as in (B). Data represent mean ± SEM, N = 10 mice, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant by one-way ANOVA. (D) Representative IF images showing eGFP expression along with neuronal (NeuN), astrocyte (GFAP), and oligodendrocyte (olig2) markers. Scale bar = 100 uM (E) Cells found to be double-positive for eGFP and one of the other markers (NeuN, GFAP, and olig2) were quantified from the IF images captured. Data presented as mean ± SEM, N = 3 mice, ∗∗∗∗ p < 0.0001 by one-way ANOVA.
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    In vivo characterization of the full-length CP040 promoter packaged in the AAVDJ capsid (A) 1 × 10 13 vg/kg of AAVDJ-CP040 -eGFP or vehicle (1x PBS) was injected into the temporal vein of <t>wild-type</t> <t>FVB/NJ</t> neonatal mice (postnatal day 1–2). Necropsies were performed on the mice at six weeks of age, and tissue samples were analyzed for vg content and eGFP transcript levels. (B) Graph presenting vg copy numbers in different organs—cortex, cerebellum, kidneys, spleen, quadriceps, spinal cord, liver and heart. (C) Graph showing the fold change in e GFP transcript levels demonstrating promoter strength in the same organs as in (B). Data represent mean ± SEM, N = 10 mice, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant by one-way ANOVA. (D) Representative IF images showing eGFP expression along with neuronal (NeuN), astrocyte (GFAP), and oligodendrocyte (olig2) markers. Scale bar = 100 uM (E) Cells found to be double-positive for eGFP and one of the other markers (NeuN, GFAP, and olig2) were quantified from the IF images captured. Data presented as mean ± SEM, N = 3 mice, ∗∗∗∗ p < 0.0001 by one-way ANOVA.
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    In vivo characterization of the full-length CP040 promoter packaged in the AAVDJ capsid (A) 1 × 10 13 vg/kg of AAVDJ-CP040 -eGFP or vehicle (1x PBS) was injected into the temporal vein of wild-type FVB/NJ neonatal mice (postnatal day 1–2). Necropsies were performed on the mice at six weeks of age, and tissue samples were analyzed for vg content and eGFP transcript levels. (B) Graph presenting vg copy numbers in different organs—cortex, cerebellum, kidneys, spleen, quadriceps, spinal cord, liver and heart. (C) Graph showing the fold change in e GFP transcript levels demonstrating promoter strength in the same organs as in (B). Data represent mean ± SEM, N = 10 mice, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant by one-way ANOVA. (D) Representative IF images showing eGFP expression along with neuronal (NeuN), astrocyte (GFAP), and oligodendrocyte (olig2) markers. Scale bar = 100 uM (E) Cells found to be double-positive for eGFP and one of the other markers (NeuN, GFAP, and olig2) were quantified from the IF images captured. Data presented as mean ± SEM, N = 3 mice, ∗∗∗∗ p < 0.0001 by one-way ANOVA.

    Journal: Molecular Therapy Advances

    Article Title: Design and initial characterization of a novel mini-promoter for gene therapies targeting the central nervous system

    doi: 10.1016/j.omta.2026.201681

    Figure Lengend Snippet: In vivo characterization of the full-length CP040 promoter packaged in the AAVDJ capsid (A) 1 × 10 13 vg/kg of AAVDJ-CP040 -eGFP or vehicle (1x PBS) was injected into the temporal vein of wild-type FVB/NJ neonatal mice (postnatal day 1–2). Necropsies were performed on the mice at six weeks of age, and tissue samples were analyzed for vg content and eGFP transcript levels. (B) Graph presenting vg copy numbers in different organs—cortex, cerebellum, kidneys, spleen, quadriceps, spinal cord, liver and heart. (C) Graph showing the fold change in e GFP transcript levels demonstrating promoter strength in the same organs as in (B). Data represent mean ± SEM, N = 10 mice, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant by one-way ANOVA. (D) Representative IF images showing eGFP expression along with neuronal (NeuN), astrocyte (GFAP), and oligodendrocyte (olig2) markers. Scale bar = 100 uM (E) Cells found to be double-positive for eGFP and one of the other markers (NeuN, GFAP, and olig2) were quantified from the IF images captured. Data presented as mean ± SEM, N = 3 mice, ∗∗∗∗ p < 0.0001 by one-way ANOVA.

    Article Snippet: Five-week-old WT FVB/NJ (stock# 001800) mice were ordered from Jackson Laboratory.

    Techniques: In Vivo, Injection, Expressing

    Schematic of the overall experiment design for promoter comparison study (A) Viral vectors used in the study. (B) Six-week-old wild-type FVB/NJ mice (C) were injected with AAVDJ-CBA-eGFP, AAVDJ-EF1α-eGFP, AAVDJ-hSYN-eGFP, AAVDJ-CP040-eGFP, or vehicle (1x PBS) through the ICV or IT route. (D and E) Mice were euthanized 9 weeks after AAV injection, and tissue analysis done for viral vg and eGFP transcript and protein analysis for promoter comparison.

    Journal: Molecular Therapy Advances

    Article Title: Design and initial characterization of a novel mini-promoter for gene therapies targeting the central nervous system

    doi: 10.1016/j.omta.2026.201681

    Figure Lengend Snippet: Schematic of the overall experiment design for promoter comparison study (A) Viral vectors used in the study. (B) Six-week-old wild-type FVB/NJ mice (C) were injected with AAVDJ-CBA-eGFP, AAVDJ-EF1α-eGFP, AAVDJ-hSYN-eGFP, AAVDJ-CP040-eGFP, or vehicle (1x PBS) through the ICV or IT route. (D and E) Mice were euthanized 9 weeks after AAV injection, and tissue analysis done for viral vg and eGFP transcript and protein analysis for promoter comparison.

    Article Snippet: Five-week-old WT FVB/NJ (stock# 001800) mice were ordered from Jackson Laboratory.

    Techniques: Comparison, Injection